CELLEXIA 2017-07-24T08:10:39+00:00

Introducing Cellexia

A high-end engineered filter product for the isolation of individual cells, such as circulating tumour cells, from blood, bodily fluids or disaggregated tissues.

Cellexia is specifically designed to capture cells for further analysis. It has a number of unique features:

  • Microlitre dead-volume to minimize sample loss.

  • Easy scalability of sample numbers via compatibility with manifold suction equipment.

  • Open configuration option enabling fast, efficient workflow.

  • Demountable structure, for flexibility of sample process requirement.

  • Torsion-free clip-fit mechanism for easy retrieval of complete filter or specific lysates.

  • Substantially greater open pore area avoids blockage compared with comparable filters.

Cellexia is created using a photolithographic process that ensures exquisite uniformity in pore size and distribution, giving it superior functional selectivity and reducing susceptibility to blockage. The engineering technology involved in the manufacturing process generates a translucent device with a precise clean edge for subsequent manipulation.

Cellexia is precision-manufactured with an integrated backing membrane for additional robustness and easy handling. The precision engineering of Cellexia is revealed by cross-sectional scanning electron microscopy.

These images, taken at the same magnification, show the striking uniformity of Cellexia (6 µm), in comparison to industry-standard membranes generated by track-etching (8 µm). The random distribution of track-etched pores results in pore coalescence, with pore sizes of up to 5 times the intended size, drastically reducing the size selectivity. Unlike track-etched membranes, Cellexia’s high precision construction ensures the highest degree of selectivity for cell separation on the basis of size.

The open area of the Cellexia filter of 27% compares favourably with an open area of just 6% for the track-etched filter, allowing more efficient filtration and less susceptibility to blockage.

The Cellexia housing is made from injection-moulded polypropylene, manufactured to the highest possible standards. The housing has female and male luer fittings, enabling either positive or negative pressure approaches to be used for sample preparation.

Cellexia-mediated filtration is very easy to undertake and the open configuration allows efficient washing to remove residual cells that might otherwise interfere with downstream analyses.

The flexible format allows an open configuration to a micro-funnel format and lends itself to solid-phase reactions such as in situ hybridization, where reagents can be applied and washed without removing the filter disc. The ability to recover cell lysates in small volumes from the upper surface of the filter membrane is conducive to molecular profiling of isolated cells by PCR or single cell genomics.

Captured cells may be evaluated for viability by staining with dye combinations to identify live and dead cells. Here, captured cells have been exposed to Acridine Orange, taken up by living cells that appear green due to the normal intracellular pH, and also to DAPI, which stains the nuclei of cells lacking an intact plasma membrane and therefore inviable.

We were so confident in Cellexia that we had the experts at Whitehouse Scientific in the UK qualify our filters. Cellexia is the only filter for circulating tumour cells that is calibrated with reference to an international standard

Please click here to view the latest Whitehouse Certificate.