Cellular Digital PCR
Enabled by Cellexia, we are developing novel, affordable, digital PCR techniques for cancer cell detection and characterisation. This technique would allow easy determination of mutations on a per cell basis so that analyses could show whether two key mutations have cropped up in a single cancer cell, indicating progress towards metastatic disease.
Digital PCR enhances the sensitivity and accuracy of molecular detection for RNA or DNA analytes (eg. somatic mutations) by allowing reaction volumes that are (eg.) 10,000 x less than a regular PCR. Under these circumstances (compartmentalization of the reaction volume) the opportunity for contamination and interference in the reaction is minimised and fewer cycles of amplification are needed to generate a signal.